Anticorps Polyclonal de lapin anti-HPSE

HPSE Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16673-1-AP

Synonymes

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

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Galerie de données de validation

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  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

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  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules DU 145
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu placentaire humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16673-1-AP cible HPSE dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPSE Protéine recombinante Ag10167
• Nom complet: heparanase
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 65 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC051321
• Symbole du gène: HPSE
• Identification du gène (NCBI): 10855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Publications

Species	Application	Title
human,mouse	WB,IF	Cell Rep; Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1.; Authors - Alex M Agelidis
mouse	WB	Diabetes Res Clin Pract; Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.; Authors - Guang Xu