Anticorps Polyclonal de lapin anti-HPS5

HPS5 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13901-1-AP

Synonymes

AIBP63, Hermansky Pudlak syndrome 5, HPS5, KIAA1017, Ru2, Ruby eye protein 2 homolog

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Galerie de données de validation

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  WB analysis of mouse liver using 13901-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13901-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 13901-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HPS5 antibody 13901-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain, tissu cutané humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

13901-1-AP cible HPS5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPS5 Protéine recombinante Ag4867
• Nom complet: Hermansky-Pudlak syndrome 5
• Masse moléculaire calculée: 1129 aa, 127 kDa
• Poids moléculaire observé: 135-141 kDa
• Numéro d’acquisition GenBank: BC033640
• Symbole du gène: HPS5
• Identification du gène (NCBI): 11234
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hermansky-Pudlak syndrome 5 protein (HPS-5), also named Ruby-eye protein 2 homolog (Ru2), plays a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5.

Publications

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
human	WB	Am J Hum Genet; A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.; Authors - Cullinane Andrew R AR
human	WB	J Invest Dermatol; Clinical, molecular, and cellular features of non-Puerto Rican Hermansky-Pudlak syndrome patients of Hispanic descent.; Authors - Carmona-Rivera Carmelo C
human	WB	Pigment Cell Melanoma Res; A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.; Authors - Cullinane Andrew R AR
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen
human	WB	Front Genet; Oculocutaneous albinism and bleeding diathesis due to a novel deletion in the HPS3 gene; Authors - Dina Marek-Yagel