Anticorps Polyclonal de lapin anti-HPS4

HPS4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14627-1-AP

Synonymes

Light-ear protein homolog, KIAA1667, Hermansky-Pudlak syndrome 4 protein, BLOC-3 complex member HPS4

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Galerie de données de validation

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  WB analysis using 14627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14627-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14627-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14627-1-AP (HPS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Hela cells using 14627-1-AP

  N/A.

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 14627-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14627-1-AP (HPS4 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 14627-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HPS4 antibody (14627-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 14627-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HPS4 Polyclonal antibody (14627-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa, résultat de l'IP de l'anti-HPS4 (14267-1-AP pour IP et détection) avec lysat de cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

14627-1-AP cible HPS4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPS4 Protéine recombinante Ag6202
• Nom complet: Hermansky-Pudlak syndrome 4
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 70-90 kDa
• Numéro d’acquisition GenBank: BC065030
• Symbole du gène: HPS4
• Identification du gène (NCBI): 89781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hermansky-Pudlak syndrome (HPS) is a genetic disease characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. HPS1 and HPS4 are the most frequently mutated genes associated with HPS in humans. Both of HPS1 and HPS4 are components of two complexes involved in biogenesis of melanosome and lysosome-related organelles: BLOC-3 and BLOC-4. HPS4 is supposed to interact with HPS1 and stabilize HPS1. The human HPS4 migrates at about 90 kDa on SDS-PAGE, versus its predicated molecular mass of 77 kDa.

Publications

Species	Application	Title
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa