Anticorps Polyclonal de lapin anti-HPS1

• Phare
• Validé par KD/KO

HPS1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15077-1-AP

Synonymes

Hermansky Pudlak syndrome 1, HPS, HPS1

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Galerie de données de validation

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  WB analysis of L02 using 15077-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15077-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15077-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15077-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15077-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 15077-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 15077-1-AP (HPS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 15077-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HPS1 antibody (15077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HEK-293 transfectées, cellules K-562, cellules L02, cellules Raji, tissu pulmonaire de souris
• Résultats positifs en IF/ICC: cellules L02, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

15077-1-AP cible HPS1 dans les applications de WB, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPS1 Protéine recombinante Ag7149
• Nom complet: Hermansky-Pudlak syndrome 1
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 70-79 kDa
• Numéro d’acquisition GenBank: BC000175
• Symbole du gène: HPS1
• Identification du gène (NCBI): 3257
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HPS1, also named as Hermansky-Pudlak syndrome 1 protein, is a 700 amino acid protein, which is a component of multiple cytoplasmic organelles. HPS1 is Apparently crucial for their normal development and function and involved in intracellular protein sorting.HPS1 is involved in the biogenesis of early melanosomes.

Publications

Species	Application	Title
mouse	WB,IF	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook ; KO Validated
		Am J Respir Cell Mol Biol; Dysregulation of Galectin-3: Implications for Hermansky-Pudlak Syndrome Pulmonary Fibrosis.; Authors - Cullinane Andrew R AR
human	RIP	Clin Exp Metastasis; Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1.; Authors - Tongxin Xu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa