Anticorps Polyclonal de lapin anti-HPRT1

• Phare
• Validé par KD/KO

HPRT1 Polyclonal Antibody for WB, IP, IHC, ELISA, IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications

30

N° de cat : 15059-1-AP

Synonymes

Hypoxanthine-guanine phosphoribosyltransferase, HPRT, HGPRTase, HGPRT, EC:2.4.2.8

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Galerie de données de validation

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  WB analysis using 15059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15059-1-AP

  WB result of HPRT1 antibody (15059-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells.

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  WB analysis of mouse liver using 15059-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15059-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15059-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15059-1-AP

  IP result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15059-1-AP (HPRT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15059-1-AP (HPRT1 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15059-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HPRT1 antibody (15059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15059-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HPRT1 (15059-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Y79, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du foie humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15059-1-AP cible HPRT1 dans les applications de WB, IP, IHC, ELISA, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPRT1 Protéine recombinante Ag7044
• Nom complet: hypoxanthine phosphoribosyltransferase 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC000578
• Symbole du gène: HPRT1
• Identification du gène (NCBI): 3251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Publications

Species	Application	Title
human	WB	J Clin Invest; Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.; Authors - Xiaoyan Yang
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
mouse	WB	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human,mouse	WB	Br J Cancer; Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells; Authors - Nabeel Kajihara
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
mouse	WB	Elife; Aging is associated with increased brain iron through cortex-derived hepcidin expression.; Authors - Tatsuya Sato