Anticorps Polyclonal de lapin anti-Haptoglobin

Haptoglobin Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 16665-1-AP

Synonymes

BP, HAPT, Haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

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Galerie de données de validation

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  WB analysis of HEK-293 using 16665-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16665-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16665-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16665-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16665-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16665-1-AP

  IP result of anti-Haptoglobin (IP:16665-1-AP, 4ug; Detection:16665-1-AP 1:500) with HepG2 cells lysate 2000ug.

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  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16665-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: sérum de souris, cellules HEK-293, plasma humain, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

16665-1-AP cible Haptoglobin dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Haptoglobin Protéine recombinante Ag10143
• Nom complet: haptoglobin
• Masse moléculaire calculée: 281aa,31 kDa; 228aa,25 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC058031
• Symbole du gène: HP
• Identification du gène (NCBI): 3240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn's disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

Publications

Species	Application	Title
	WB	Circ Res; Haptoglobin enhances cardiac transplant rejection.; Authors - Hua Shen
	WB	Theranostics; Interleukin-6 Induced "Acute" Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics.; Authors - Ting Xue
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
		Antioxid Redox Signal; Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate.; Authors - John D Belcher
human	WB	World J Gastroenterol; N-linked glycoproteomic profiling in esophageal squamous cell carcinoma; Authors - Qi-Wei Liu
human	IHC	Oncol Rep; Proteomic-based analysis for identification of potential serum biomarkers in gallbladder cancer.; Authors - Tan Yi Y