Anticorps Polyclonal de lapin anti-HORMAD1

HORMAD1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 13917-1-AP

Synonymes

Cancer/testis antigen 46, CT46, HORMA domain containing 1, HORMAD1, Newborn ovary HORMA protein, NOHMA, RP11 363I22.1

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Galerie de données de validation

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  WB analysis using 13917-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 13917-1-AP (HORMAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 17 publications below
• CoIP: See 1 publications below

Informations sur le produit

13917-1-AP cible HORMAD1 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HORMAD1 Protéine recombinante Ag4914
• Nom complet: HORMA domain containing 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC047406
• Symbole du gène: HORMAD1
• Identification du gène (NCBI): 84072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.

Publications

Species	Application	Title
mouse	IF	Sci Adv; SKP1 drives the prophase I to metaphase I transition during male meiosis.; Authors - Yongjuan Guan
human,mouse	WB,IF,CoIP	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
mouse	IF	Curr Biol; Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes.; Authors - Tanmoy Bhattacharyya
mouse	WB,IF	PLoS Genet; Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis.; Authors - Fukuda Tomoyuki T
mouse	WB,IF	PLoS Genet; Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase.; Authors - Yasuhiro Fujiwara