Anticorps Polyclonal de lapin anti-HOPX

• Phare
• Validé par KD/KO

HOPX Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (2)

N° de cat : 11419-1-AP

Synonymes

CAMEO, HOD, Homeodomain only protein, HOP, HOP homeobox, HOPX, LAGY, NECC1, OB1, Odd homeobox protein 1, SMAP31, TOTO

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Galerie de données de validation

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  WB analysis using 11419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 11419-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 11419-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu d'intestin grêle de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu placentaire humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 19 publications below

Informations sur le produit

11419-1-AP cible HOPX dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOPX Protéine recombinante Ag1979
• Nom complet: HOP homeobox
• Masse moléculaire calculée: 73 aa, 8 kDa
• Poids moléculaire observé: 8-12 kDa
• Numéro d’acquisition GenBank: BC014225
• Symbole du gène: HOPX
• Identification du gène (NCBI): 84525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOPX (Homeodomain-only protein) gene has various synonyms including HOD, HOP, OB1, LAGY and NECC1. The protein encoded by this gene is an unusual homeodomain protein that lacks certain conserved residues required for DNA binding. HOPX has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. HOPX protein acts as an antagonist to the serum response factor (SRF), which regulates the opposing processes of cell proliferation and differentiation. Overexpression of HOPX causes cardiac hypertrophy. HOPX protein can inhibit SRF-dependent transcriptional activation by recruiting histone deacetylase (HDAC) activity.

Publications

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	IF	Cell; Origins and Proliferative States of Human Oligodendrocyte Precursor Cells.; Authors - Wei Huang
human	IHC	Cancer Cell; Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.; Authors - Lindsay M LaFave
human		Cancer Cell; Control of alveolar differentiation by the lineage transcription factors GATA6 and HOPX inhibits lung adenocarcinoma metastasis.; Authors - William K C Cheung
human	IF	Cell Stem Cell; LIF signaling regulates outer radial glial to interneuron fate during human cortical development; Authors - Madeline G Andrews
mouse	IF	Protein Cell; BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic period; Authors - Zhenmeiyu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ana (Verified Customer) (11-10-2020)

We recently published with this paper: PubMed ID: 32819430 Images are within the manuscript.Protocol information: We used Citirc Acid Buffer Antigen Retrieval

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human Brain (fetal), Paraffin Processed

HOPX Antibody Immunohistochemistry validation (1:250 dilution) in Human Brain (fetal), Paraffin Processed (Cat no:11419-1-AP)

FH

Nicole (Verified Customer) (07-23-2019)

Used to mark stem cell cultures for differentiation into neural like cells

• Applications: Immunohistochemistry, Immunofluorescence,
• Cell Tissue Type: Stem Cell culture