Anticorps Polyclonal de lapin anti-HOMER3

• Phare
• Validé par KD/KO

HOMER3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16624-1-AP

Synonymes

HOMER 3, homer homolog 3 (Drosophila), Homer protein homolog 3, HOMER3, VESL3

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Galerie de données de validation

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  WB analysis of mouse brain using 16624-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16624-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16624-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16624-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cervelet humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1200
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

16624-1-AP cible HOMER3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOMER3 Protéine recombinante Ag9923
• Nom complet: homer homolog 3 (Drosophila)
• Masse moléculaire calculée: 361 aa, 40 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC012113
• Symbole du gène: HOMER3
• Identification du gène (NCBI): 9454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
rat	WB	Neuroscience; Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.; Authors - Dan Liu
human	IHC	Cell Death Dis; Long non-coding RNA HOMER3-AS1 drives hepatocellular carcinoma progression via modulating the behaviors of both tumor cells and macrophages.; Authors - Jian Pu ; KD Validated