Anticorps Polyclonal de lapin anti-HOMER1

• Phare
• Validé par KD/KO

HOMER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 12433-1-AP

Synonymes

HOMER1A, Homer-1, Homer protein homolog 1, homer homolog 1 (Drosophila), Homer 1

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Galerie de données de validation

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  WB analysis of rat brain using 12433-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:5000 incubated at room temperature for 1 hour.

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  WB analysis of human/mouse brain using 12433-1-AP

  WB result of anti-HOMER1 (12433-1-ap) with mouse brain lysate and human brain lysate.

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  WB analysis of human brain using 12433-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12433-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 12433-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of fetal human brain using 12433-1-AP

  IP result of anti-HOMER1 (IP:12433-1-AP, 4ug; Detection:12433-1-AP 1:1000) with fetal human brain tissue lysate 4000 ug.

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  IHC staining of human gliomas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 12433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 12433-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HOMER1 antibody (12433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 12433-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 12433-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human HOMER1 (12433-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cerveau humain/de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral humain fœtal,
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de rat, tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat,
• Résultats positifs en IF/ICC: cellules PC-12, cellules MCF-7
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

12433-1-AP cible HOMER1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HOMER1 Protéine recombinante Ag3109
• Nom complet: homer homolog 1 (Drosophila)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 46-48 kDa
• Numéro d’acquisition GenBank: BC015502
• Symbole du gène: HOMER1
• Identification du gène (NCBI): 9456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse	WB	Elife; Pathway-specific dysregulation of striatal excitatory synapses by LRRK2 mutations.; Authors - Chuyu Chen
mouse	IF	PLoS Genet; Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.; Authors - Maha El Shahawy
rat	IF	Aging (Albany NY); Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation.; Authors - Xiangrong Cui ; KD Validated
Mouse, Rat	WB	CNS Neurosci Ther; Acid-sensing Ion Channels Activation and Hypoxia Upregulate Homer1a Expression.; Authors - Jing-Jing Su
mouse	WB,IF	Cell Signal; Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c.; Authors - Su Jingjing J