Anticorps Polyclonal de lapin anti-HNRNPUL1

• Phare
• Validé par KD/KO

HNRNPUL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10578-1-AP

Synonymes

E1B AP5, E1BAP5, HNRNPUL1, HNRPUL1

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Galerie de données de validation

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  WB analysis of HEK-293 using 10578-1-AP

  WB result of HNRNPUL1 antibody (10578-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPUL1 transfected HEK-293 cells.

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  WB analysis of A549 using 10578-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10578-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10578-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10578-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10578-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10578-1-AP

  IP Result of anti-HNRNPUL1 (IP:10578-1-AP, 5ug; Detection:10578-1-AP 1:2000) with HeLa cells lysate 2000ug.

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  IHC staining of human ovary tumor using 10578-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HepG2 using 10578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10578-1-AP

  Immunofluorescent analysis of HepG2 cells, using HNRNPUL1 antibody 10578-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 10578-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug HNRNPUL1 antibody (10578-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules MCF-7, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10578-1-AP cible HNRNPUL1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPUL1 Protéine recombinante Ag0899
• Nom complet: heterogeneous nuclear ribonucleoprotein U-like 1
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 90-115 kDa
• Numéro d’acquisition GenBank: BC002564
• Symbole du gène: HNRNPUL1
• Identification du gène (NCBI): 11100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .

Publications

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB,IF,IP	Proc Natl Acad Sci U S A; Influenza virus targets the mRNA export machinery and the nuclear pore complex.; Authors - Satterly Neal N
	WB	PLoS Pathog; Cellular RNA Binding Proteins NS1-BP and hnRNP K Regulate Influenza A Virus RNA Splicing.; Authors - Tsai Pei-Ling PL
human	WB,IF	EMBO Rep; Vesicular stomatitis virus inhibits mitotic progression and triggers cell death.; Authors - Chakraborty Papia P
mouse	WB,IF	J Autoimmun; RNA-binding protein hnRNP UL1 binds κB sites to attenuate NF-κB-mediated inflammation.; Authors - Zhongfei Ma ; KO Validated
human	IHC	Exp Cell Res; HNRNPUL1 inhibits cisplatin sensitivity of esophageal squamous cell carcinoma through regulating the formation of circMAN1A2.; Authors - Juan Li