Anticorps Polyclonal de lapin anti-HNRNPU

HNRNPU Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 14599-1-AP

Synonymes

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

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Galerie de données de validation

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  WB analysis using 14599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14599-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14599-1-AP

  IP result of anti-HNRNPU (IP:14599-1-AP, 4ug; Detection:14599-1-AP 1:800) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 14599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (14599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• RIP: See 3 publications below

Informations sur le produit

14599-1-AP cible HNRNPU dans les applications de WB, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPU Protéine recombinante Ag6141
• Nom complet: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• Masse moléculaire calculée: 825 aa, 91 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC003621
• Symbole du gène: HNRNPU
• Identification du gène (NCBI): 3192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB,IP	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li
human	WB,RIP	Cancer Res; DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.; Authors - Songge Xing
human	WB	Elife; Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction; Authors - Amy E Spens
human	WB	Hum Mol Genet; Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.; Authors - Hiroaki Suzuki