Anticorps Polyclonal de lapin anti-HNRNPL

• Phare
• Validé par KD/KO

HNRNPL Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18354-1-AP

Synonymes

hnRNP L, HNRNPL, HNRPL, P/OKcl.14

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Galerie de données de validation

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  WB analysis using 18354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18354-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 18354-1-AP

  IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18354-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18354-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPL antibody (18354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules PC-3, cellules RAW 264.7, tissu hépatique de souris
• Résultats positifs en IP: cellules BxPC-3,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

18354-1-AP cible HNRNPL dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Humain, porc, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPL Protéine recombinante Ag13211
• Nom complet: heterogeneous nuclear ribonucleoprotein L
• Masse moléculaire calculée: 572 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC069184
• Symbole du gène: HNRNPL
• Identification du gène (NCBI): 3191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.

Publications

Species	Application	Title
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	ChIP	RNA Biol; CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.; Authors - Yi Ran
human	WB	J Proteome Res; C1QBP Negatively Regulates the Activation of Oncoprotein YBX1 in the Renal Cell Carcinoma as Revealed by Interactomics Analysis.; Authors - Yong Wang
pig,bovine,hamster	WB,IF	J Virol; Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5' Untranslated Region.; Authors - Chao Sun ; KO Validated
human	WB	Methods; Fast and unbiased purification of RNA-protein complexes after UV cross-linking.; Authors - Erika C Urdaneta
human	IHC	DNA Cell Biol; HNRNPL Is Identified and Validated as a Prognostic Biomarker Associated with Microsatellite Instability in Human Gastric Cancer.; Authors - Xiongjie Yu