Anticorps Polyclonal de lapin anti-HNRNPK

• Phare
• Validé par KD/KO

HNRNPK Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (1)

N° de cat : 11426-1-AP

Synonymes

CSBP, hnRNP K, HNRNPK, HNRPK, TUNP

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Galerie de données de validation

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  WB analysis of MCF-7 using 11426-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11426-1-AP (HNRNPK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11426-1-AP

  IP result of anti-HNRNPK (IP:11426-1-AP, 4ug; Detection:11426-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human breast cancer using 11426-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11426-1-AP (HNRNPK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11426-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11426-1-AP (HNRNPK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPK antibody (11426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPK antibody (11426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11426-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HNRNPK (11426-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 7 publications below

Informations sur le produit

11426-1-AP cible HNRNPK dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPK Protéine recombinante Ag1976
• Nom complet: heterogeneous nuclear ribonucleoprotein K
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC014980
• Symbole du gène: HNRNPK
• Identification du gène (NCBI): 3190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability.

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
		Nat Commun; PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.; Authors - Chen-Yu Wang
		Nucleic Acids Res; Capturing RNA-protein interaction via CRUIS.; Authors - Ziheng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

DEBJIT (Verified Customer) (09-17-2020)

WB: 1:2000IP: 2ug/mg cell lysate

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: Endothelial cells