Anticorps Polyclonal de lapin anti-HNRNPD

• Phare
• Validé par KD/KO

HNRNPD Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 12770-1-AP

Synonymes

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

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Galerie de données de validation

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  WB analysis using 12770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12770-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 12770-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (12770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12770-1-AP cible HNRNPD dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPD Protéine recombinante Ag3458
• Nom complet: heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
• Masse moléculaire calculée: 355 aa, 38 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC026015
• Symbole du gène: HNRNPD
• Identification du gène (NCBI): 3184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Publications

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	IHC	Brain; Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.; Authors - Johnathan Cooper-Knock
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	EMBO Rep; Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates.; Authors - Johan Aarum