Anticorps Polyclonal de lapin anti-HNRNPA3

• Phare
• Validé par KD/KO

HNRNPA3 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 25142-1-AP

Synonymes

HNRPA3, HNRNPA 3, hnRNP A3, FBRNP

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Galerie de données de validation

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  WB analysis of Jurkat using 25142-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25142-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 25142-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse colon using 25142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200, E-cadherin antibody (20874-1-AP, Magenta), CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange).

• 

  IF Staining of HeLa using 25142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules Jurkat
• Résultats positifs en IF-P: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

25142-1-AP cible HNRNPA3 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPA3 Protéine recombinante Ag18248
• Nom complet: heterogeneous nuclear ribonucleoprotein A3
• Masse moléculaire calculée: 378 aa, 40 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC113470
• Symbole du gène: HNRNPA3
• Identification du gène (NCBI): 220988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.

Publications

Species	Application	Title
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
		Arterioscler Thromb Vasc Biol; Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.; Authors - Kenneth Stapleton
mouse	IP,IF,IHC	Neurobiol Dis; ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.; Authors - Haiyan An
human	WB,IF	Sci China Life Sci; Identification of mecciRNAs and their roles in the mitochondrial entry of proteins.; Authors - Xu Liu
mouse	WB	Mediators Inflamm; Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages.; Authors - John E Wiktorowicz

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-21-2023)

Works well in ICC and WB in human cells. Also gives a weak but specific signal in the mouse CNS tissue (spinal cord). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa ICC: 5% goat serum in PBST; mainly nuclear IHC: 4% PFA fixed and paraffinised sections; standard antigen retrieval and DAB detection; mainly nuclear Used at 1:1000, with incubation overnight at 4C, in all applications

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells; mouse / spinal cord

HNRNPA3 Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells; mouse / spinal cord (Cat no:25142-1-AP)