Anticorps Polyclonal de lapin anti-HNRNPA0

HNRNPA0 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 10848-1-AP

Synonymes

hnRNP A0, HNRNPA0, HNRPA0

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Galerie de données de validation

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  WB analysis using 10848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10848-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500.

• 

  IF Staining of HeLa using 10848-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10848-1-AP (HNRNPA0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10848-1-AP cible HNRNPA0 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPA0 Protéine recombinante Ag1312
• Nom complet: heterogeneous nuclear ribonucleoprotein A0
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC019271
• Symbole du gène: HNRNPA0
• Identification du gène (NCBI): 10949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Publications

Species	Application	Title
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	IF	Cell; RNA damage compartmentalization by DHX9 stress granules; Authors - Yilong Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaochen (Verified Customer) (07-31-2023)

hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.

• Applications: Western Blot

HNRNPA0 Antibody Western Blot validation ( dilution) in (Cat no:10848-1-AP)