Anticorps Polyclonal de lapin anti-HMMR-Specific

• Phare
• Validé par KD/KO

HMMR-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 15820-1-AP

Synonymes

CD168, HMMR, HMMR-Specific, IHABP, RHAMM

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Galerie de données de validation

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  WB analysis using 15820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15820-1-AP (HMMR-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15820-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR-Specific antibody (15820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15820-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human HMMR-Specific (15820-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules C6, cellules K-562, cellules T-47D
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

15820-1-AP cible HMMR-Specific dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: hyaluronan-mediated motility receptor (RHAMM)
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: NM_001142556
• Symbole du gène: HMMR
• Identification du gène (NCBI): 3161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMMR, also named RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus playing roles in wound healing and tumor metastasis. Remarkably different molecular masses of RHAMM have been published. Antibodies raised against RHAMM have been reported to recognize proteins of 56, 66 and 70 kDa in the supernatant of murine 3T3 fibroblasts(PMID: 2440472). Some of the isoforms detectable by anti-RHAMM antibodies, including the 52 kDa form and a 48-49 kDa form found in 10T1/2 cells, have been speculated to be underglycosylated precursor forms of the mature RHAMM protein(PMID: 1705559).

Publications

Species	Application	Title
mouse	FC	Nature; Tracing haematopoietic stem cell formation at single-cell resolution.; Authors - Fan Zhou
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
rat	IF	Int J Mol Sci; Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat; Authors - Jian-He Lu
human	WB	Int J Biol Sci; Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.; Authors - Deyu Zhang ; KD Validated
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Heriberto (Verified Customer) (11-28-2018)

Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .

• Applications: Western Blot,
• Primary Antibody Dilution: Try 1:1000, 1:750 and 1:500
• Cell Tissue Type: SN12L1, SN12C and ACHN- Renal carcinoma cell lines

HMMR-Specific Antibody Western Blot, validation (Try 1:1000, 1:750 and 1:500 dilution) in SN12L1, SN12C and ACHN- Renal carcinoma cell lines (Cat no:15820-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C