Anticorps Polyclonal de lapin anti-HMMR-Specific

• Phare
• Validé par KD/KO

HMMR-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 15820-1-AP

Synonymes

HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein

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Galerie de données de validation

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  WB analysis using 15820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15820-1-AP (HMMR-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15820-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR-Specific antibody (15820-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HepG2 using 15820-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human HMMR-Specific (15820-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules C6, cellules K-562, cellules T-47D
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15820-1-AP cible HMMR-Specific dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: hyaluronan-mediated motility receptor (RHAMM)
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: NM_001142556
• Symbole du gène: HMMR
• Identification du gène (NCBI): 3161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)

Publications

Species	Application	Title
mouse	FC	Nature; Tracing haematopoietic stem cell formation at single-cell resolution.; Authors - Fan Zhou
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
rat	IF	Int J Mol Sci; Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat; Authors - Jian-He Lu
human	WB	Int J Biol Sci; Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.; Authors - Deyu Zhang ; KD Validated
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Acta Biochim Biophys Sin (Shanghai); FOXM1 mediates methotrexate resistance in osteosarcoma cells by promoting autophagy; Authors - Luoyang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Heriberto (Verified Customer) (11-28-2018)

Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .

• Applications: Western Blot,
• Primary Antibody Dilution: Try 1:1000, 1:750 and 1:500
• Cell Tissue Type: SN12L1, SN12C and ACHN- Renal carcinoma cell lines

HMMR-Specific Antibody Western Blot, validation (Try 1:1000, 1:750 and 1:500 dilution) in SN12L1, SN12C and ACHN- Renal carcinoma cell lines (Cat no:15820-1-AP)

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