Anticorps Polyclonal de lapin anti-HMGN2

HMGN2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10953-1-AP

Synonymes

HMG17, HMGN2

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 10953-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10953-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10953-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10953-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10953-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10953-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules HL-60, cellules K-562
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

10953-1-AP cible HMGN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGN2 Protéine recombinante Ag1401
• Nom complet: high-mobility group nucleosomal binding domain 2
• Masse moléculaire calculée: 9 aa, 3 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC014644
• Symbole du gène: HMGN2
• Identification du gène (NCBI): 3151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
human	ELISA	Mol Cancer; HMGN2, a new anti-tumor effector molecule of CD8+ T cells.; Authors - Lin Su
pig	WB	J Proteomics; DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase; Authors - Chen Zhou