Anticorps Polyclonal de lapin anti-HMGN1

HMGN1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11695-1-AP

Synonymes

HMG14, HMGN1

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Galerie de données de validation

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  WB analysis of HepG2 using 11695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11695-1-AP

  Immunofluorescent analysis of HepG2 cells, using HMGN1 antibody 11695-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

11695-1-AP cible HMGN1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGN1 Protéine recombinante Ag2315
• Nom complet: high-mobility group nucleosome binding domain 1
• Masse moléculaire calculée: 100 aa, 11 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC023984
• Symbole du gène: HMGN1
• Identification du gène (NCBI): 3150
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly

Publications

Species	Application	Title
human	WB	J Exp Med; High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses.; Authors - Yang De D
mouse	WB	Nat Commun; Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.; Authors - Bingfeng Zuo
human	WB	Cancer Res; The alarmin HMGN1 contributes to anti-tumor immunity and is a potent immunoadjuvant.; Authors - Feng Wei
mouse	WB	Theranostics; Alarmin augments the antitumor immunity of lentiviral vaccine in ectopic, orthotopic and autochthonous hepatocellular carcinoma mice.; Authors - Zhili Liu
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
mouse	IHC	PeerJ; PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice; Authors - Yuecheng Jiang