• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-HMGCS1

HMGCS1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 17643-1-AP

Synonymes

HMG CoA synthase, HMGCS, HMGCS1



Applications testées

Résultats positifs en WBcellules A431, cellules A549, cellules HepG2, cellules LNCaP, tissu hépatique de souris
Résultats positifs en IPtissu hépatique de souris
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17643-1-AP cible HMGCS1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HMGCS1 Protéine recombinante Ag11825
Nom complet 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
Masse moléculaire calculée 520 aa, 57 kDa
Poids moléculaire observé 57 kDa
Numéro d’acquisition GenBankBC083514
Symbole du gène HMGCS1
Identification du gène (NCBI) 3157
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).

Protocole

Product Specific Protocols
WB protocol for HMGCS1 antibody 17643-1-APDownload protocol
IF protocol for HMGCS1 antibody 17643-1-APDownload protocol
IP protocol for HMGCS1 antibody 17643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis

Authors - Wei Zhou
  • KD Validated
humanWB

Nat Cancer

Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism

Authors - Linjie Zhao
  • KD Validated
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouse,humanIF,WB

J Cell Biol

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Authors - Wan Yun Ho
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
humanWB

Mol Cell Proteomics

Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.

Authors - Saraon Punit P

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

K (Verified Customer) (12-20-2020)

Used for western blotting (1:1000) and IHC (1:200), worked well for me

  • Applications: Western Blot
  • Cell Tissue Type: mouse liver tissues
FH

H (Verified Customer) (07-09-2020)

This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T and HCT116
HMGCS1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:17643-1-AP)
FH

Boyan (Verified Customer) (07-08-2019)

This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.

  • Applications: Western Blot,
  • Cell Tissue Type: MEF