Anticorps Polyclonal de lapin anti-HMGCR

• Phare
• Validé par KD/KO

HMGCR Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (1)

N° de cat : 13533-1-AP

Synonymes

HMG CoA reductase, HMGCR

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Galerie de données de validation

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  IHC staining of human liver cancer using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13533-1-AP (HMGCR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13533-1-AP (HMGCR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 10x lens)..

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  IHC staining of human small intestine using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 40x lens)..

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 9 publications below
• IF: See 3 publications below

Informations sur le produit

13533-1-AP cible HMGCR dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGCR Protéine recombinante Ag4444
• Nom complet: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• Masse moléculaire calculée: 888 aa, 95 kDa
• Numéro d’acquisition GenBank: BC033692
• Symbole du gène: HMGCR
• Identification du gène (NCBI): 3156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGCR(3-hydroxy-3-methylglutaryl-coenzyme A reductase) is also named as HMG-CoA reductase and belongs to the HMG-CoA reductase family. This protein exists as a 97-kDa glycoprotein in the endoplasmic reticulum (ER) and is the rate-determining enzyme of the biosynthesis pathway(PMID:15830340).The ectopic expression of either full-length HMGCR or its novel splice variant promotes dysregulation of the MVA pathway. And the ectopic expression of cHMGCR-FL(60 kDa) and cHMGCR-D13(55 kDa) can be detected in HepG2 cells(PMID:20696928).This protein is also can exsit as a oligomer(PMID:20696928).

Publications

Species	Application	Title
mouse	IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	IF	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho ; KD Validated
human	IHC	Acta Pharmacol Sin; Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.; Authors - Hai-Yan Wang ; KD Validated
human		Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
human		PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	IHC	Mol Metab; LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway.; Authors - Ziye Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Malak (Verified Customer) (11-30-2020)

The antibody works very well in IHC staining especially at pH6 and with 1/100 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Hepatic tissue