Anticorps Polyclonal de lapin anti-HMG20A

• Phare
• Validé par KD/KO

HMG20A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12085-2-AP

Synonymes

high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

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Galerie de données de validation

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  WB analysis of HEK-293 using 12085-2-AP

  WB result of HMG20A antibody (12085-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20A transfected HEK-293 cells.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12085-2-AP

  IP result of anti-HMG20A (IP:12085-2-AP, 4ug; Detection:12085-2-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12085-2-AP (HMG20A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12085-2-AP cible HMG20A dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMG20A Protéine recombinante Ag2718
• Nom complet: high-mobility group 20A
• Masse moléculaire calculée: 347 aa, 40 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC021959
• Symbole du gène: HMG20A
• Identification du gène (NCBI): 10363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation

Publications

Species	Application	Title
human	WB,IP	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther ; KD Validated
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
mouse	WB	Cancer Cell Int; Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.; Authors - Yu Fu
human	IHC	Biochim Biophys Acta; Interactome of the inhibitory isoform of the nuclear transporter Importin 13.; Authors - Shadma Fatima
mouse	WB,IHC,IF,IP	Biochim Biophys Acta; The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.; Authors - Davies Rebecca G RG