Anticorps Polyclonal de lapin anti-HKR1

HKR1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 14902-1-AP

Synonymes

GLI Kruppel family member HKR1, HKR1, Protein HKR1

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Galerie de données de validation

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  WB analysis of COLO 320 using 14902-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 14902-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14902-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14902-1-AP cible HKR1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HKR1 Protéine recombinante Ag6694
• Nom complet: GLI-Kruppel family member HKR1
• Masse moléculaire calculée: 73 kDa, 75 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC053845
• Symbole du gène: HKR1
• Identification du gène (NCBI): 284459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HKR1, also named as ZNF875, belongs to the krueppel C2H2-type zinc-finger protein family. HRK1 is a modular protein that comprises a photosensory rhodopsin, a histidine kinase, a response regulator and a putative guanylyl cyclase domain. HKR1 is related to the protonation state of the Schiff base function that provides the linkage of the retinal chromophore with a Lys side chain of the protein (PMID: 25836735). HRK1 has 2 isoforms with the molecular mass of 73-75 kDa.