Anticorps Polyclonal de lapin anti-Histone H2A type 3

• Phare
• Validé par KD/KO

Histone H2A type 3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10445-1-AP

Synonymes

HIST3H2A, histone, histone cluster 3, H2a, Histone H2A, Histone H2A type 3

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Galerie de données de validation

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  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10445-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (Histone H2A type 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10445-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10445-1-AP (HIST3H2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10445-1-AP cible Histone H2A type 3 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2A type 3 Protéine recombinante Ag0374
• Nom complet: histone cluster 3, H2a
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-18 kDa
• Numéro d’acquisition GenBank: BC001193
• Symbole du gène: Histone H2A type 3
• Identification du gène (NCBI): 92815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone H2A type 3, it is expected to be located in the nucleus, and the protein is enriched in the brain tissue. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. The molecular weight of Histone H2A type 3 is 14 kDa.

Publications

Species	Application	Title
human,mouse	WB,IP,IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su ; KO Validated
mouse	WB	Nucleic Acids Res; Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.; Authors - Tianjin Shen
mouse	IHC	EMBO Rep; MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors.; Authors - Hongyan Ma
human	WB	BMC Cancer; HIST3H2A promotes the progression of prostate cancer through inhibiting cell necroptosis; Authors - Lihong Yang