Anticorps Polyclonal de lapin anti-Histone H1.2

Histone H1.2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15446-1-AP

Synonymes

H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d

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Galerie de données de validation

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  WB analysis of Jurkat using 15446-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15446-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15446-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 15446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15446-1-AP (Histone H1.2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules L02, cellules MCF-7, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

15446-1-AP cible Histone H1.2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H1.2 Protéine recombinante Ag7607
• Nom complet: histone cluster 1, H1c
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 32-33 kDa
• Numéro d’acquisition GenBank: BC002649
• Symbole du gène: Histone H1.2
• Identification du gène (NCBI): 3006
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Nat Commun; Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.; Authors - Chen Xu
human	WB	Cell Physiol Biochem; Tumor-Secreted Exosomal miR-222 Promotes Tumor Progression via Regulating P27 Expression and Re-Localization in Pancreatic Cancer.; Authors - Zhonghu Li
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang
human	WB	Oxid Med Cell Longev; Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.; Authors - Reiko Iida