Anticorps Polyclonal de lapin anti-HEXA

• Phare
• Validé par KD/KO

HEXA Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 11317-1-AP

Synonymes

HEXA, Hexosaminidase subunit A, TSD

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Galerie de données de validation

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  WB analysis using 11317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11317-1-AP (HEXA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11317-1-AP (HEXA antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 11317-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 11317-1-AP (HEXA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7, cellules PC-3
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

11317-1-AP cible HEXA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HEXA Protéine recombinante Ag1854
• Nom complet: hexosaminidase A (alpha polypeptide)
• Masse moléculaire calculée: 529 aa, 60 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC018927
• Symbole du gène: HEXA
• Identification du gène (NCBI): 3073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-hexosaminidase subunit alpha (HEXA) is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Publications

Species	Application	Title
mouse	WB	Immunity; The phagosomal proteome in interferon-gamma-activated macrophages.; Authors - Matthias Trost
mouse	IF	J Immunol; VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation.; Authors - Pushparaj Peter N PN
human	IHC,IF,WB	Front Oncol; Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.; Authors - Mengxian Jia ; KD Validated
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland
human	IHC	Proc Natl Acad Sci U S A; A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB; Authors - Yuqi Lin
mouse,rat	IF	Nat Commun; Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents; Authors - Yachao He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (01-26-2023)

Did not detect expected mature HEXA ~54kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: HEK293T

HEXA Antibody Western Blot validation (1:20,000 dilution) in HEK293T (Cat no:11317-1-AP)

FH

Georgina (Verified Customer) (09-15-2022)

Good to have the chance to buy a sample to try out an experiment. The antibody worked.

• Applications: Western Blot