Anticorps Polyclonal de lapin anti-HECTD1

• Phare
• Validé par KD/KO

HECTD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 20605-1-AP

Synonymes

E3 ligase for inhibin receptor, EULIR, HECT domain containing 1, HECTD1, KIAA1131

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Galerie de données de validation

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  WB analysis using 20605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20605-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20605-1-AP

  Immunofluorescent analysis of Hela cells, using HECTD1 antibody 20605-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

20605-1-AP cible HECTD1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HECTD1 Protéine recombinante Ag14581
• Nom complet: HECT domain containing 1
• Masse moléculaire calculée: 2612 aa, 290 kDa
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: BC006237
• Symbole du gène: HECTD1
• Identification du gène (NCBI): 25831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

Publications

Species	Application	Title
human	WB	Mol Cell; Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation.; Authors - Wenbo Li
mouse	WB	J Neurosci; Circular RNA DLGAP4 ameliorates ischemic stroke outcomes by targeting miR-143 to regulate endothelial-mesenchymal transition associated with blood-brain barrier integrity.; Authors - Ying Bai
mouse	IF,WB	Cell Biosci; Involvement of HECTD1 in LPS-induced astrocyte activation via σ-1R-JNK/p38-FOXJ2 axis.; Authors - Ying Tang ; KO Validated
mouse	WB	Cell Microbiol; The serine/threonine protein kinase of Streptococcus suis serotype 2 affects the ability of the pathogen to penetrate the blood brain barrier.; Authors - Liu Rui
human	WB	Hum Cell; Nucleoporin 93, a new substrate of the E3 ubiquitin protein ligase HECTD1, promotes esophageal squamous cell carcinoma progression; Authors - Jinfeng Zhang
human	WB	Sci Rep; Proteasomal turnover of the RhoGAP tumor suppressor DLC1 is regulated by HECTD1 and USP7.; Authors - Yannick Frey ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nick (Verified Customer) (04-20-2022)

Excellent stuff. Highly recommend.

• Applications: Western Blot, Immunoprecipitation