Anticorps Polyclonal de lapin anti-HDAC4

• Phare
• Validé par KD/KO

HDAC4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 17449-1-AP

Synonymes

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

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Galerie de données de validation

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  WB analysis using 17449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17449-1-AP

  IP result of anti-HDAC4 (IP:17449-1-AP, 4ug; Detection:17449-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 17449-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

17449-1-AP cible HDAC4 dans les applications de WB, IHC, IF-P, IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDAC4 Protéine recombinante Ag11572
• Nom complet: histone deacetylase 4
• Masse moléculaire calculée: 972 aa, 106 kDa
• Poids moléculaire observé: 119-140 kDa
• Numéro d’acquisition GenBank: BC039904
• Symbole du gène: HDAC4
• Identification du gène (NCBI): 9759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Publications

Species	Application	Title
	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
human	WB, IF	Hepatology; Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1.; Authors - Lucía Barbier-Torres ; KD Validated
	WB,IHC,IF	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang ; KD Validated
human	ChIP,CoIP	Mol Ther Nucleic Acids; Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity.; Authors - Qianqian Guo
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated
human	WB	J Am Heart Assoc; Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells.; Authors - Yanjiao Li