Anticorps Polyclonal de lapin anti-HDAC3

• Phare
• Validé par KD/KO

HDAC3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

44

N° de cat : 10255-1-AP

Synonymes

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Galerie de données de validation

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  WB analysis of U-251 using 10255-1-AP

  WB result of HDAC3 antibody (10255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3 transfected U-251 cells.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10255-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10255-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10255-1-AP

  IP Result of anti-HDAC3 (IP:10255-1-AP, 3ug; Detection:10255-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of K-562 using 10255-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HDAC3 antibody (10255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules U-251
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 37 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

10255-1-AP cible HDAC3 dans les applications de WB, IP, IHC, IF, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDAC3 Protéine recombinante Ag0396
• Nom complet: histone deacetylase 3
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC000614
• Symbole du gène: HDAC3
• Identification du gène (NCBI): 8841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
human	WB	Mol Ther; Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis; Authors - Chao Li
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
		Oncogene; miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.; Authors - X-H He