Anticorps Polyclonal de lapin anti-HDAC10

HDAC10 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 24913-1-AP

Synonymes

HD10, HDAC10, histone deacetylase 10

---

Galerie de données de validation

• 

  WB analysis of fetal human brain using 24913-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 24913-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 24913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (24913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

24913-1-AP cible HDAC10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDAC10 Protéine recombinante Ag18470
• Nom complet: histone deacetylase 10
• Masse moléculaire calculée: 669 aa, 71 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC125083
• Symbole du gène: HDAC10
• Identification du gène (NCBI): 83933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Publications

Species	Application	Title
mouse, rat	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	IHC	Oncol Lett; HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.; Authors - Xiangxiang Tao
human	WB	Cell Death Dis; WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.; Authors - Yingtong Feng
human	IHC,WB	J Cell Mol Med; RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming; Authors - Dali Yan
human	WB,IHC	Discov Oncol; Elevated histone deacetylase 10 expression promotes the progression of clear cell renal cell carcinoma by Notch-1-PTEN signaling axis; Authors - Bin Zheng