Anticorps Polyclonal de lapin anti-HBS1L

• Phare
• Validé par KD/KO

HBS1L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10359-1-AP

Synonymes

EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

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Galerie de données de validation

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  WB analysis of Jurkat using 10359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10359-1-AP

  WB result of HBS1L antibody (10359-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

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  WB analysis of Jurkat using 10359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10359-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10359-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10359-1-AP

  IP result of anti-HBS1L (IP:10359-1-AP, 4ug; Detection:10359-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 10359-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10359-1-AP (HBS1L antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HepG2 using 10359-1-AP

  Immunofluorescent analysis of HepG2 cells, using HBS1L antibody 10359-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A2780, cellules HEK-293, cellules SKOV-3, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below

Informations sur le produit

10359-1-AP cible HBS1L dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HBS1L Protéine recombinante Ag0388
• Nom complet: HBS1-like (S. cerevisiae)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC001465
• Symbole du gène: HBS1L
• Identification du gène (NCBI): 10767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

Publications

Species	Application	Title
human	WB	Mol Cell; Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.; Authors - Michael K Slevin
mouse	WB	Elife; Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.; Authors - Markus Terrey
human	WB	Biochem J; Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.; Authors - John Rp Knight
human	WB	J Biol Chem; RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.; Authors - Hussein H Aly ; KD Validated
mouse	WB	PLoS Genet; Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.; Authors - Amy E O'Connell ; KO Validated
mouse	WB	Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu ; KO Validated