Anticorps Polyclonal de lapin anti-HAS3

• Phare
• Validé par KD/KO

HAS3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15609-1-AP

Synonymes

HA synthase 3, HAS3, hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3

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Galerie de données de validation

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  WB analysis using 15609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15609-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15609-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules L02, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

15609-1-AP cible HAS3 dans les applications de WB, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAS3 Protéine recombinante Ag7999
• Nom complet: hyaluronan synthase 3
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC021853
• Symbole du gène: HAS3
• Identification du gène (NCBI): 3038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HAS3(hyaluronan synthase 3) belongs to the NodC/HAS family, which are widely expressed in human tissues and appear to function in embryogenesis, wound healing and other processes associated with cellular proliferation and migration(PMID:14566823). HAS3 is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. It may play a role in the progression of colon cancer and, as such, may provide novel targets for diagnostic and therapeutic interventions(PMID:14566823). It has 2 isoforms produced by alternative splicing and the protein has a glycosylation site.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human,mouse	WB,IHC	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB	Int J Mol Sci; Elevation of Hyaluronan Synthase by Magnesium Supplementation Mediated through the Activation of GSK3 and CREB in Human Keratinocyte-Derived HaCaT Cells.; Authors - Kana Marunaka
human	WB,IHC,FC	Cancers (Basel); C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.; Authors - Romana Vidergar
		Invest Ophthalmol Vis Sci; PMID: 27092721; Authors - Anny M S Cheng
human	WB	Mol Med Rep; miR‑29a‑3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels.; Authors - Feihu Bai ; KD Validated