Anticorps Polyclonal de lapin anti-H6PD

• Phare
• Validé par KD/KO

H6PD Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15255-1-AP

Synonymes

6PGL, G6PDH, GDH, H6PD

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Galerie de données de validation

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  WB analysis of HepG2 using 15255-1-AP

  WB result of H6PD antibody (15255-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-H6PD transfected HepG2 cells.

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  WB analysis of HepG2 using 15255-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15255-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15255-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15255-1-AP

  IP result of anti-H6PD (IP:15255-1-AP, 4ug; Detection:15255-1-AP 1:1000) with HepG2 cells lysate 1600ug.

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  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15255-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15255-1-AP cible H6PD dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: H6PD Protéine recombinante Ag7109
• Nom complet: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89-95 kDa
• Numéro d’acquisition GenBank: BC081559
• Symbole du gène: H6PD
• Identification du gène (NCBI): 9563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD.

Publications

Species	Application	Title
rat	IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human,mouse	WB	iScience; A fingerprint of 2-[18F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[18F]FDG-6-P could affect tracer accumulation; Authors - Eva-Maria Patronas ; KD Validated
	WB	Biomolecules; PMID: 31480692; Authors - Xixi Guo