Anticorps Polyclonal de lapin anti-Histone H3.3

• Phare
• Validé par KD/KO

Histone H3.3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13754-1-AP

Synonymes

H3 histone, family 3A, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, histone, Histone H3.3

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Galerie de données de validation

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  WB analysis using 13754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of mouse kidney using 13754-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13754-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (Histone H3.3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13754-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13754-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13754-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13754-1-AP (H3F3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13754-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 13754-1-AP (Histone H3.3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, tissu de thymus de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

13754-1-AP cible Histone H3.3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H3.3 Protéine recombinante Ag4696
• Nom complet: H3 histone, family 3A
• Masse moléculaire calculée: 136 aa, 15 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC038989
• Symbole du gène: Histone H3
• Identification du gène (NCBI): 3020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].

Publications

Species	Application	Title
human	WB	Int J Mol Sci; ADAM10 Plasma and CSF Levels Are Increased in Mild Alzheimer's Disease.; Authors - Izabela Pereira Vatanabe
human	WB	Biochem Biophys Res Commun; Protective effects of 4-octyl itaconate against inflammatory response in angiotensin II-induced oxidative stress in human primary retinal pigment epithelium.; Authors - Xinyu Fu
rat	IF	Fish Shellfish Immunol; Triclocarban evoked neutrophil extracellular trap formation in common carp (Cyprinus carpio L.) by modulating SIRT3-mediated ROS crosstalk with ERK1/2/p38 signaling; Authors - Siwen Li
human	WB	Nat Commun; Impaired histone inheritance promotes tumor progression; Authors - Congcong Tian
human	WB	Clin Transl Med; LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A; Authors - Danlei Chen ; KD Validated
		J Hepatol; IL-6/STAT3 axis dictates the PNPLA3-mediated susceptibility to non-alcoholic fatty liver disease; Authors - Jiwoon Park