Anticorps Polyclonal de lapin anti-Glypican 3

Glypican 3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 25175-1-AP

Synonymes

DGSX, Glypican 3, GPC3, GTR2 2, Intestinal protein OCI 5, MXR7, OCI 5, OCI5, SDYS, SGB, SGBS, SGBS1

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Galerie de données de validation

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  WB analysis of HepG2 using 25175-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25175-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

25175-1-AP cible Glypican 3 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glypican 3 Protéine recombinante Ag10129
• Nom complet: glypican 3
• Masse moléculaire calculée: 580 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC035972
• Symbole du gène: GPC3
• Identification du gène (NCBI): 2719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).

Publications

Species	Application	Title
mouse		Hepatology; The Mutational Features of Aristolochic Acid-Induced Mouse and Human Liver Cancers.; Authors - Zhao-Ning Lu
human	IF	Acta Biomater; Enhanced glypican-3-targeted identification of hepatocellular carcinoma with liver fibrosis by pre-degrading excess fibrotic collagen; Authors - Jiaming Zhuo
human		Cancer Res; hPCL3s promotes hepatocellular carcinoma metastasis by activating β-catenin signaling.; Authors - Zhen Cai
mouse	WB	Redox Biol; Butaselen prevents hepatocarcinogenesis and progression through inhibiting thioredoxin reductase activity.; Authors - Xiaoqing Zheng
mouse	WB	Cancers (Basel); Therapeutic Adenovirus Vaccine Combined Immunization with IL-12 Induces Potent CD8+ T Cell Anti-Tumor Immunity in Hepatocellular Carcinoma; Authors - Samuel C Mok
human	WB	Front Oncol; TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.; Authors - Meng Wang