Anticorps Polyclonal de lapin anti-GTSE1

• Phare
• Validé par KD/KO

GTSE1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 21319-1-AP

Synonymes

B99, G 2 and S phase expressed 1, GTSE 1, GTSE1, Protein B99 homolog

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Galerie de données de validation

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  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21319-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse spleen using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules L02, cellules MCF-7, cellules SH-SY5Y, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

21319-1-AP cible GTSE1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTSE1 Protéine recombinante Ag13497
• Nom complet: G-2 and S-phase expressed 1
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 66-77 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC006325
• Symbole du gène: GTSE1
• Identification du gène (NCBI): 51512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

Publications

Species	Application	Title
human	IHC	Mod Pathol; Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets.; Authors - Ellinor Andersson
human	WB	Mol Ther Nucleic Acids; lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation.; Authors - Xuelei Ruan
human	IHC	Cancer Sci; High G2 and S-phase expressed 1 expression promotes acral melanoma progression and correlates with poor clinical prognosis.; Authors - Tianxiao Xu
human,mouse	WB,IHC	Lab Invest; GTSE1 promotes prostate cancer cell proliferation via the SP1/FOXM1 signaling pathway.; Authors - Wenjie Lai ; KD Validated
human	WB,IHC	Lab Invest; GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma.; Authors - Weihao Chen ; KD Validated
human	WB,IHC,IF	Mol Carcinog; G2 and S phase-expressed-1 induces chromosomal instability in esophageal squamous cell carcinoma cells and inhibits cell apoptosis through ROS/JNK signaling; Authors - Man Zhang ; KD Validated