Anticorps Polyclonal de lapin anti-GTPBP4

• Phare
• Validé par KD/KO

GTPBP4 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 13897-1-AP

Synonymes

CRFG, GTP binding protein 4, GTP binding protein NGB, GTPBP4, NGB, NOG1

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Galerie de données de validation

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  WB analysis using 13897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13897-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13897-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13897-1-AP

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IF Staining of HeLa using 13897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13897-1-AP (GTPBP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu rénal humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13897-1-AP cible GTPBP4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTPBP4 Protéine recombinante Ag4859
• Nom complet: GTP binding protein 4
• Masse moléculaire calculée: 634 aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC038975
• Symbole du gène: GTPBP4
• Identification du gène (NCBI): 23560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.

Publications

Species	Application	Title
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
Drosophila	WB,IF	Proc Natl Acad Sci U S A; A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.; Authors - Lunardi Andrea A ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis.; Authors - Haitao Yu ; KD Validated
human	WB	Biomed Res Int; Determining the Clinical Value and Critical Pathway of GTPBP4 in Lung Adenocarcinoma Using a Bioinformatics Strategy: A Study Based on Datasets from The Cancer Genome Atlas.; Authors - Zhiqian Zhang ; KD Validated
human	WB	Cell Rep; UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer; Authors - Meng Pan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

kaosheng (Verified Customer) (11-11-2019)

This antibody detects a very clear and specific band in polysome profiling fractions of human TF1 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: TF1