Anticorps Polyclonal de lapin anti-GTF2IRD1

GTF2IRD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 17052-1-AP

Synonymes

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

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Galerie de données de validation

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  WB analysis of HeLa using 17052-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17052-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17052-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17052-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17052-1-AP

  IP result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17052-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17052-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17052-1-AP cible GTF2IRD1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTF2IRD1 Protéine recombinante Ag10718
• Nom complet: GTF2I repeat domain containing 1
• Masse moléculaire calculée: 959 aa, 106 kDa
• Poids moléculaire observé: 145 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC018136
• Symbole du gène: GTF2IRD1
• Identification du gène (NCBI): 9569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.