Anticorps Polyclonal de lapin anti-GTF2H2

GTF2H2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16005-1-AP

Synonymes

BTF2, BTF2 p44, BTF2P44, GTF2H2, T BTF2P44, TFIIH

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Galerie de données de validation

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  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16005-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16005-1-AP (GTF2H2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16005-1-AP cible GTF2H2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTF2H2 Protéine recombinante Ag8653
• Nom complet: general transcription factor IIH, polypeptide 2, 44kDa
• Masse moléculaire calculée: 395aa,44 kDa; 165aa,19 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC005345
• Symbole du gène: GTF2H2
• Identification du gène (NCBI): 2966
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.

Publications

Species	Application	Title
human	WB	Cell; CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.; Authors - Ashleigh E Schaffer
human	WB	Tissue Cell; Exosome-derived GTF2H2 from Huh7 cells can inhibit endothelial cell viability, migration, tube formation, and permeability; Authors - Zhenkun Li