Anticorps Polyclonal de lapin anti-GSTA2

GSTA2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10505-1-AP

Synonymes

Glutathione S transferase A2, GST class alpha member 2, GST gamma, GST HA subunit 2, GST2, GSTA2, GSTA2 2, GTA2, GTH2

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Galerie de données de validation

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  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

10505-1-AP cible GSTA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTA2 Protéine recombinante Ag0781
• Nom complet: glutathione S-transferase alpha 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC002895
• Symbole du gène: GSTA2
• Identification du gène (NCBI): 2939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
rat	WB	Antioxidants (Basel); Isatin Counteracts Diethylnitrosamine/2-Acetylaminofluorene-Induced Hepatocarcinogenesis in Male Wistar Rats by Upregulating Anti-Inflammatory, Antioxidant, and Detoxification Pathways.; Authors - Nagwa G Tawfik
mouse	WB	Eur J Nutr; Multi-omics integration reveals the hepatoprotective mechanisms of ursolic acid intake against chronic alcohol consumption.; Authors - Xin Yan
human	WB	Kidney Blood Press Res; Identification of PDK4 as hub gene for diabetic nephropathy using co-expression network analysis; Authors - Yuanyuan Han
mouse	WB	J Pharmacol Sci; Enhanced expression of glutathione S-transferase in the hippocampus following acute treatment with trimethyltin in vivo.; Authors - Nagashima Reiko R
mouse	WB	J Toxicol Pathol; Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.; Authors - Shirai Makoto M

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (02-27-2019)

This antibody works best at 1:500 in TBST milk with O/N incubation. I get beautiful blot with the use of this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells

GSTA2 Antibody Western Blot, validation (1:500 dilution) in Fallopian tube epithelial cells (Cat no:10505-1-AP)