Anticorps Polyclonal de lapin anti-GSR

• Phare
• Validé par KD/KO

GSR Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 18257-1-AP

Synonymes

GLUR, glutathione reductase, GR, GRase, GRD1, GSR

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Galerie de données de validation

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  WB analysis of HeLa using 18257-1-AP

  WB result of GSR antibody (18257-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSR transfected HeLa cells.

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  WB analysis using 18257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18257-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18257-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18257-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18257-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18257-1-AP

  IP result of anti-GSR (IP:18257-1-AP, 4ug; Detection:18257-1-AP 1:500) with HeLa cells lysate 2600ug.

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSR antibody (18257-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu placentaire humain, tissu pulmonaire de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below

Informations sur le produit

18257-1-AP cible GSR dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSR Protéine recombinante Ag13080
• Nom complet: glutathione reductase
• Masse moléculaire calculée: 522 aa, 56 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC069244
• Symbole du gène: GSR
• Identification du gène (NCBI): 2936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

Publications

Species	Application	Title
human	WB	Redox Biol; Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.; Authors - Angel Gallego-Selles
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
human	WB	Nutrients; A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle; Authors - Miriam Martinez-Canton
human	WB	Pharmacol Res; Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.; Authors - Meng-Ting Chang
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	Free Radic Biol Med; Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.; Authors - Jan Blecha