Anticorps Polyclonal de lapin anti-GSK3B

• Phare
• Validé par KD/KO

GSK3B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

332

Avis (1)

N° de cat : 22104-1-AP

Synonymes

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β

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Galerie de données de validation

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  WB analysis using 22104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22104-1-AP

  WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis of mouse testis using 22104-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22104-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of mouse kidney using 22104-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 22104-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 22104-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22104-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22104-1-AP

  IP result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22104-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 22104-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules LNCaP, cellules PC-3, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de rat, tissu de thymus de souris, tissu ovarien de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de côlon de rat, tissu d'hyperplasie de la prostate humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 321 publications below
• IHC: See 15 publications below
• IF: See 10 publications below
• IP: See 5 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

22104-1-AP cible GSK3B dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSK3B Protéine recombinante Ag17320
• Nom complet: glycogen synthase kinase 3 beta
• Masse moléculaire calculée: 433 aa, 48 kDa
• Poids moléculaire observé: 46-48 kDa
• Numéro d’acquisition GenBank: BC000251
• Symbole du gène: GSK3B
• Identification du gène (NCBI): 2932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha
human	WB	Mol Cancer; Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1.; Authors - Shan Xing
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
zebrafish	WB	Nat Commun; ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.; Authors - Xiaoyun Sun
rat	WB	Hepatology; Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.; Authors - Daimin Xiang
rat	WB	Theranostics; A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair; Authors - Zhiyuan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GSK3B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:22104-1-AP)