Anticorps Polyclonal de lapin anti-GSDMD

• Phare
• Validé par KD/KO

GSDMD Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

228

Avis (1)

N° de cat : 20770-1-AP

Synonymes

Gasdermin-D, C-terminal, Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D

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Galerie de données de validation

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20770-1-AP

  WB result of GSDMD antibody (20770-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSDMD transfected Jurkat cells.

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 20770-1-AP

  IP result of anti-GSDMD (IP:20770-1-AP, 4ug; Detection:20770-1-AP 1:4000) with THP-1 cells lysate 1560 ug.

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  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 20770-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human GSDMD (20770-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu hépatique de rat
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 184 publications below
• IHC: See 47 publications below
• IF: See 56 publications below
• FC: See 1 publications below

Informations sur le produit

20770-1-AP cible GSDMD dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSDMD Protéine recombinante Ag14757
• Nom complet: gasdermin D
• Masse moléculaire calculée: 484 aa, 53 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC008904
• Symbole du gène: GSDMD
• Identification du gène (NCBI): 79792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Publications

Species	Application	Title
human	WB	Nature; Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.; Authors - Xing Liu ; KD Validated
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang
human	WB	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
Human	WB	Cell Res; Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.; Authors - Wan-Ting He
human	WB	J Exp Med; Protein kinase D at the Golgi controls NLRP3 inflammasome activation.; Authors - Zhirong Zhang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Isha (Verified Customer) (03-02-2021)

Bands were so clear. Could not refrain myself from writing this review.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice kidney

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