Anticorps Polyclonal de lapin anti-GSDMB

GSDMB Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12885-1-AP

Synonymes

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

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Galerie de données de validation

• 

  WB analysis of PC-3 using 12885-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12885-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12885-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12885-1-AP cible GSDMB dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSDMB Protéine recombinante Ag3895
• Nom complet: gasdermin B
• Masse moléculaire calculée: 411 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC025682
• Symbole du gène: GSDMB
• Identification du gène (NCBI): 55876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB, IHC	Int J Biol Sci; USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.; Authors - Haiqing He
human	IHC	Front Oncol; Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.; Authors - Jia You
human	WB	Toxicol Appl Pharmacol; Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity; Authors - Anping Song
human	IHC,IF	Cell Death Dis; Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence; Authors - Ying Zhang
human	WB	J Allergy Clin Immunol; A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.; Authors - Ronald A Panganiban