Anticorps Polyclonal de lapin anti-GREM2

GREM2 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13892-1-AP

Synonymes

CKTSF1B2, DAN domain family member 3, DAND3, GREM2, Gremlin 2, PRDC

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Galerie de données de validation

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  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13892-1-AP (GREM2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13892-1-AP (GREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 13892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using GREM2 antibody (13892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu ovarien de souris, tissu ovarien humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris, tissu ovarien de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13892-1-AP cible GREM2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GREM2 Protéine recombinante Ag4854
• Nom complet: gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
• Masse moléculaire calculée: 168 aa, 19 kDa
• Numéro d’acquisition GenBank: BC046632
• Symbole du gène: GREM2
• Identification du gène (NCBI): 64388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Am J Pathol; The Onset of Heat-Induced Testicular Calcification in Mice: Involvement of the Telomeric Locus on Chromosome 1.; Authors - Masataka Chihara
human	IF	Stem Cells Dev; Coordinated proliferation and differentiation of human iPS cell-derived cardiac progenitor cells depends on BMP signaling regulation by Gremlin 2.; Authors - Jeffery Bylund
mouse	IHC	Andrology; Genetic factors derived from the MRL/MpJ mouse function to maintain the integrity of spermatogenesis after heat exposure.; Authors - M Chihara
mouse	WB,IHC	Metabolism; SGLT2 inhibitor empagliflozin downregulates miRNA-34a-5p and targets GREM2 to inactivate hepatic stellate cells and ameliorate non-alcoholic fatty liver disease-associated fibrosis; Authors - Yunfeng Shen