Anticorps Polyclonal de lapin anti-GRASP65,GORASP1

GRASP65,GORASP1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10747-2-AP

Synonymes

Golgi phosphoprotein 5, GOLPH5, GORASP1, GRASP65, GRASP65,GORASP1, P65

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Galerie de données de validation

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  WB analysis using 10747-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10747-2-AP (GRASP65,GORASP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10747-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GRASP65,GORASP1 antibody (10747-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10747-2-AP cible GRASP65,GORASP1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRASP65,GORASP1 Protéine recombinante Ag1054
• Nom complet: golgi reassembly stacking protein 1, 65kDa
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC008928
• Symbole du gène: GRASP65
• Identification du gène (NCBI): 64689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)

Publications

Species	Application	Title
human	WB	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human	IF	J Cell Biochem; Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury.; Authors - Guang Ji
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
human	WB	Biochem Biophys Res Commun; Golgi damage caused by dysfunction of PiT-2 in primary familial brain calcification; Authors - Huifang Sun
mouse	WB	Cell Death Dis; GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response; Authors - Theodomir Dusabimana