Anticorps Polyclonal de lapin anti-GPX5

GPX5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18731-1-AP

Synonymes

EGLP, Glutathione peroxidase 5, GPx 5, GPX5, GSHPx 5

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Galerie de données de validation

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  WB analysis of mouse testis using 18731-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18731-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 18731-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18731-1-AP (GPX5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules PC-3, tissu hépatique de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

18731-1-AP cible GPX5 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutathione peroxidase 5 (epididymal androgen-related protein)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 22-28 kDa
• Numéro d’acquisition GenBank: NM_001509
• Symbole du gène: GPX5
• Identification du gène (NCBI): 2880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

Publications

Species	Application	Title
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
mouse	IF	Sci Rep; Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.; Authors - Lin Huang
mouse	WB	Mol Biol Rep; Autophagy-related 7 proteindependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell; Authors - Fanhong Lin
pig	WB	Animals (Basel); Effect of Boar Sperm Proteins and Quality Changes on Field Fertility.; Authors - Ilias Michos
pig	WB	Theriogenology; Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17 C.; Authors - Weike Shaoyong
human	WB	Anim Reprod Sci; Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.; Authors - Ingrid Vilagran