Anticorps Polyclonal de lapin anti-GPX4

GPX4 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

125

N° de cat : 14432-1-AP

Synonymes

GPX4

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Galerie de données de validation

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  WB analysis using 14432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14432-1-AP (GPX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14432-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPX4 antibody (14432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 113 publications below
• IHC: See 24 publications below
• IF: See 17 publications below
• CoIP: See 2 publications below

Informations sur le produit

14432-1-AP cible GPX4 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPX4 Protéine recombinante Ag5809
• Nom complet: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC022071
• Symbole du gène: GPX4
• Identification du gène (NCBI): 2879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

Publications

Species	Application	Title
human	WB	Drug Resist Updat; Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance; Authors - Xu Lin
mouse	WB	Biomaterials; Tumor microenvironment-initiated lipid redox cycling for efficient triple-negative breast cancer therapy; Authors - Tian-Jiao Zhou
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
mouse	IHC	Drug Des Devel Ther; Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides; Authors - Xuedong Yin
mouse	WB	Drug Des Devel Ther; Ketamine Attenuates Airway Inflammation via Inducing Inflammatory Cells Apoptosis and Activating Nrf2 Pathway in a Mixed-Granulocytic Murine Asthma Model; Authors - Shilin Xiao
mouse	WB	Mater Today Bio; In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy.; Authors - Jiehan Li