Anticorps Polyclonal de lapin anti-GPR37/Pael-R

• Phare
• Validé par KD/KO

GPR37/Pael-R Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14820-1-AP

Synonymes

EDNRBL, ETBR LP 1, GPR37, GPR37/Pael-R, hET(B)R LP, PAELR, Pael-R

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Galerie de données de validation

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  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14820-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3200
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below

Informations sur le produit

14820-1-AP cible GPR37/Pael-R dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPR37/Pael-R Protéine recombinante Ag6591
• Nom complet: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC040007
• Symbole du gène: GPR37
• Identification du gène (NCBI): 2861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).

Publications

Species	Application	Title
human	WB	Mol Syst Biol; Systematic protein-protein interaction mapping for clinically relevant human GPCRs.; Authors - Kate Sokolina
mouse	WB	Proc Natl Acad Sci U S A; GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.; Authors - Meyer Rebecca C RC
human	WB	Oncogene; GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and histone lactylation via Hippo pathway; Authors - Jiamin Zhou
mouse,human	WB,IF	EMBO Rep; Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6.; Authors - Birgit S Berger ; KD Validated
rat	WB	Glia; Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37.; Authors - Beihui Liu
mouse	WB	Biomedicines; Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages.; Authors - Zhengjiang Qian ; KO Validated